Gene Editing Technologies: CRISPR/Cas9 and Beyond for Genetic Disease Therapy and Research
DOI:
https://doi.org/10.36676/ssjmra.v1.i4.26Keywords:
Gene, Technologies, CRISPR/Cas9, Genetic Disease, TherapyAbstract
Among these, CRISPR/Cas9 stands out as a versatile tool for precisely targeting and modifying specific sequences within the genome. This paper provides an overview of CRISPR/Cas9 and other emerging gene editing technologies, discussing their potential applications in treating genetic diseases and advancing scientific research. Additionally, ethical considerations and challenges associated with gene editing are explored, along with future directions for this rapidly evolving field.
References
Doudna, Jennifer A., and Emmanuelle Charpentier. "The new frontier of genome engineering with CRISPR-Cas9." Science 346, no. 6213 (2014): 1258096.
Komor, Alexis C., Yongjoo B. Kim, Michael S. Packer, John A. Zuris, and David R. Liu. "Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage." Nature 533, no. 7603 (2016): 420-424.
Adli, Mazhar. "The CRISPR tool kit for genome editing and beyond." Nature Communications 9, no. 1 (2018): 1911.
Cyranoski, David. "CRISPR gene-editing tested in a person for the first time." Nature News 539, no. 7630 (2016): 479.
Liang, Xue, Haowen Potter, Bo Liu, Yongxing Wang, Shuangping Xu, and Feng Zhang. "Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection." Journal of Biotechnology 208 (2015): 44-53.
Gaj, Thomas, Christopher A. Gersbach, and Carlos F. Barbas III. "ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering." Trends in Biotechnology 31, no. 7 (2013): 397-405.
Nelson, Christopher E., Kristopher L. Nazor, Ciaran Lee, and Louise C. Laurent. "Institutional Oversight of Human Gene Editing." Cell Stem Cell 18, no. 3 (2016): 277-281.
Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell 157, no. 6 (2014): 1262-1278.
Khalil, Ahmad M., and Charles A. Rupprecht. "Gene therapy for inherited diseases." Human Molecular Genetics 25, no. R1 (2016): R94-R100.
Chandrasegaran, Srinivasan, and Francisco J. Mojica. "Applications of CRISPR-Cas systems in biology and medicine." The FEBS Journal 283, no. 17 (2016): 3167-3179.
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2024 Shodh Sagar Journal for Medical Research Advancement
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.